We share R tutorials from scientists at academic and scientific institutions with a goal to give everyone in the world access to a free knowledge. Our tutorials cover different topics including statistics, data manipulation and visualization!

Basic Statistics

To conduct a one-sample *t*-test in R, we use the syntax `t.test(y, mu = 0)`

where `x`

is the name of our variable of interest and `mu`

is set equal to the mean specified by the null hypothesis.

So, for example, if we wanted to test whether the volume of a shipment of lumber was less than usual (\(\mu_0=39000\) cubic feet), we would run:

set.seed(0) treeVolume <- c(rnorm(75, mean = 36500, sd = 2000)) t.test(treeVolume, mu = 39000) # Ho: mu = 39000One Sample t-test data: treeVolume t = -12.2883, df = 74, p-value < 2.2e-16 alternative hypothesis: true mean is not equal to 39000 95 percent confidence interval: 36033.60 36861.38 sample estimates: mean of x 36447.49

With these simulated data, we see that the current shipment of lumber has a significantly lower volume than we usually see:

t = -12.2883, p-value < 2.2e-16

To conduct a paired-samples test, we need either two vectors of data, \(y_1\) and \(y_2\), or we need one vector of data with a second that serves as a binary grouping variable. The test is then run using the syntax `t.test(y1, y2, paired=TRUE)`

.

For instance, let’s say that we work at a large health clinic and we’re testing a new drug, Procardia, that’s meant to reduce hypertension. We find 1000 individuals with a high systolic blood pressure (\(\bar{x}=145\)mmHg, \(SD=9\)mmHg), we give them Procardia for a month, and then measure their blood pressure again. We find that the mean systolic blood pressure has decreased to 138mmHg with a standard deviation 8mmHg.

We can visualize this difference with a kernel density plot as:

Here, we would conduct a t-test using:

set.seed(2820) preTreat <- c(rnorm(1000, mean = 145, sd = 9)) postTreat <- c(rnorm(1000, mean = 138, sd = 8)) t.test(preTreat, postTreat, paired = TRUE)Paired t-test data: preTreat and postTreat t = 19.7514, df = 999, p-value < 2.2e-16 alternative hypothesis: true difference in means is not equal to 0 95 percent confidence interval: 6.703959 8.183011 sample estimates: mean of the differences 7.443485

Again, we see that there is a statistically significant difference in means on

t = 19.7514, p-value < 2.2e-16

The independent-samples test can take one of three forms, depending on the structure of your data and the equality of their variances. The general form of the test is `t.test(y1, y2, paired=FALSE)`

. By default, R assumes that the variances of `y1`

and `y2`

are unequal, thus defaulting to Welch's test. To toggle this, we use the flag `var.equal=TRUE`

.

In the three examples shown here we’ll test the hypothesis that Clevelanders and New Yorkers spend different amounts monthly eating out. The first example assumes that we have two numeric vectors: one with Clevelanders' spending and one with New Yorkers' spending. The second example uses a binary grouping variable with a single column of spending data. (That is, there is only one column of spending data; however, for each dollar amount, the next column specifies whether it is for a New Yorker or a Clevelander.) Finally, the third example assumes that the variances of the two samples are unequal and uses Welch's test.

Independent-samples t-test where y1 and y2 are numeric:

set.seed(0) ClevelandSpending <- rnorm(50, mean = 250, sd = 75) NYSpending <- rnorm(50, mean = 300, sd = 80) t.test(ClevelandSpending, NYSpending, var.equal = TRUE)Two Sample t-test data: ClevelandSpending and NYSpending t = -3.6361, df = 98, p-value = 0.0004433 alternative hypothesis: true difference in means is not equal to 0 95 percent confidence interval: -77.1608 -22.6745 sample estimates: mean of x mean of y 251.7948 301.7125

Where y1 is numeric and y2 is binary:

spending <- c(ClevelandSpending, NYSpending) city <- c(rep("Cleveland", 50), rep("New York", 50)) t.test(spending ~ city, var.equal = TRUE)Two Sample t-test data: spending by city t = -3.6361, df = 98, p-value = 0.0004433 alternative hypothesis: true difference in means is not equal to 0 95 percent confidence interval: -77.1608 -22.6745 sample estimates: mean in group Cleveland mean in group New York 251.7948 301.7125

With equal variances not assumed:

t.test(ClevelandSpending, NYSpending, var.equal = FALSE)Welch Two Sample t-test data: ClevelandSpending and NYSpending t = -3.6361, df = 97.999, p-value = 0.0004433 alternative hypothesis: true difference in means is not equal to 0 95 percent confidence interval: -77.1608 -22.6745 sample estimates: mean of x mean of y 251.7948 301.7125

In each case, we see that the results really don’t differ substantially: our simulated data show that in any case New Yorkers spend more each month at restaurants than Clevelanders do. However, should you want to test for equality of variances in your data prior to running an independent-samples t-test, R offers an easy way to do so with the `var.test()`

function:

var.test(ClevelandSpending, NYSpending)F test to compare two variances data: ClevelandSpending and NYSpending F = 1.0047, num df = 49, denom df = 49, p-value = 0.9869 alternative hypothesis: true ratio of variances is not equal to 1 95 percent confidence interval: 0.5701676 1.7705463 sample estimates: ratio of variances 1.004743

Have questions? Post a comment below! Or download the full code used in this example.